RNA-Seq of Phaseolus vulgaris: BAT 477 leaf after 150 min of stress - SRA

SRX1890864: RNA-Seq of Phaseolus vulgaris: BAT 477 leaf after 150 min of stress
1 ILLUMINA (Illumina HiSeq 2000) run: 15.3M spots, 2.2G bases, 1.2Gb downloads

Design: Fresh tissue was sampled from 15 days seedlings. The total RNA were extracted using a commercial PureLink RNA Mini Kit. The RNA-Seq library was prepared using Illumina TruSeq RNA kit and paired-end sequenced using Illumina HiSeq 2000. CASAVA 1.8.2 was used to generate the FASTQ files and trimmomatic was used for quality control.

Submitted by: Brazilian Agricultural Research Corporation - EMBRAPA Rice and Bean - Embrapa Rice and Beans

Study: Phaseolus vulgaris Transcriptome in drought conditions

PRJNA327176 • SRP077562 • All experiments • All runs

show Abstracthide Abstract

This experiment comprises RNA sequencing (RNA-Seq) of two genotypes of common bean (Phaseolus vulgaris L.), BAT 477 and Pérola, that have contrasting performance under drought stress. Both genotypes have Mesoamerican origin and were grown in a controlled environment (hydroponics system) for growth and induction of drought stress. The main objective of this project was to characterize the gene expression profiles of the described genotypes in response to drought stress conditions. Sequencing was performed on the platform Illumina Genome Analyzer IIx, paired-end reads with 100pb. The data were previously treated for quality assurance of the deposited reads.

Sample:

SAMN05323720 • SRS1530080 • All experiments • All runs

Organism: Phaseolus vulgaris

Library:

Name: HiSeq_BAT_150_L

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 15.3M spots, 2.2G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR3735343	15,345,762	2.2G	1.2Gb	2017-12-01

ID:: 2737064

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity